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HA Tag Antibody
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS: 25
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b |
| Clone/Animal Names | 12CA5 |
| Other Names | Tag from influenza hemagglutinin protein |
|---|---|
| Target/Specificity | KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen. |
| Dilution | WB~~1:8000 E~~Use at an assay dependent concentration. |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HA Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Provided below are standard protocols that you may find useful for product applications.
Background
Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.
References
Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.
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